Pericyte stem cells induce Ly6G+ cell accumulation and immunotherapy resistance in pancreatic cancer

ABSTRACT: We report the identification of a cell population that shares pericyte, stromal and stemness features, does not harbour the KrasG12D mutation and drives tumoral growth in vitro and in vivo. We term these cells pericyte stem cells (PeSCs) and define them as CD45-EPCAM-CD29+CD106+CD24+CD44+ cells. We perform studies with p48-Cre;KrasG12D (KC), pdx1-Cre;KrasG12D;Ink4a/Arffl/fl (KIC) and pdx1-Cre;KrasG12D;p53R172H (KPC) and tumor tissues from PDAC and chronic pancreatitis patients. We also perform single cell RNAseq analysis and reveal a unique signature of PeSC. Under steady-state conditions, PeSCs are barely detectable in the pancreas but present in the neoplastic microenvironment both in humans and mice. The co-injection of PeSCs and tumor epithelial cells leads to increased tumor growth, d

SUBMITTER: Dr. Zhichong Wu 

PROVIDER: S-SCDT-10_15252-EMBR_202256524 | biostudies-other |

REPOSITORIES: biostudies-other