Tissue-like Environments Shape Functional Interactions of HIV-1 with Immature Dendritic Cells

ABSTRACT: Immature Dendritic Cells (iDCs) migrate in microenvironments with distinct cell and extracellular matrix densities in vivo and contribute to HIV-1 dissemination and mounting of antiviral immune responses. Here, we find that, compared to standard 2D suspension cultures, 3D collagen as tissue-like environment alters iDC properties and their response to HIV-1 infection. iDCs adopt an elongated morphology with increased deformability in 3D collagen at unaltered activation, differentiation, cytokine secretion or responsiveness to LPS. While 3D collagen reduces HIV-1 particle uptake by iDCs, fusion efficiency is increased to elevate productive infection rates due to elevated cell surface exposure of the HIV-1 binding receptor DC-SIGN. In contrast, 3D collagen reduces HIV transfer to CD4 T-cells

SUBMITTER: Lara Gallucci 

PROVIDER: S-SCDT-10_15252-EMBR_202356818 | biostudies-other |

REPOSITORIES: biostudies-other