Tetherin antagonism by SARS-CoV-2 ORF3a and Spike protein enhances virus release

ABSTRACT: The antiviral restriction factor, tetherin, blocks the release of several different families of enveloped viruses, including the Coronaviridae. Tetherin is an interferon-induced protein that forms parallel homodimers between the host cell and viral particles, linking viruses to the surface of infected cells and inhibiting their release. We demonstrate that SARS-CoV-2 infection causes tetherin downregulation, and that tetherin depletion from cells enhances SARS-CoV-2 viral titres. We investigate the potential viral proteins involved in abrogating tetherin function and find that SARS-CoV-2 ORF3a reduces tetherin localisation within biosynthetic organelles where Coronaviruses bud, and increases tetherin localisation to late endocytic organelles via reduced retrograde recycling. We also find t

SUBMITTER: Dr. Hazel Stewart 

PROVIDER: S-SCDT-10_15252-EMBR_202357224 | biostudies-other |

REPOSITORIES: biostudies-other