Genome-wide association and environmental suppression of the mortal germline phenotype of wild C. elegans

ABSTRACT: The animal germline lineage needs to be maintained along generations. However, some Caenorhabditis elegans wild isolates display a mortal germline phenotype, leading to sterility after several generations at 25{degree sign}C. Using a genome-wide association approach, we detect a significant peak on chromosome III around 5 Mb, confirmed by introgressions. Thus a seemingly deleterious genotype is maintained at intermediate frequency in the species. Environmental rescue is a likely explanation and indeed associated bacteria and microsporidia suppress the phenotype of wild isolates as well as mutants in small RNA inheritance (nrde-2) and histone modifications (set-2). Escherichia coli strains of the K-12 lineage suppress the phenotype compared to B strains. By shifting a wild strain from E. co

SUBMITTER: Lise Frézal 

PROVIDER: S-SCDT-10_15252-EMBR_202358116 | biostudies-other |

REPOSITORIES: biostudies-other