MiR-182-3p targets TRF2 and impairs tumor growth of triple negative breast cancer

ABSTRACT: The Telomeric Repeat-binding Factor 2 (TRF2) is a telomere-capping protein that plays a key role in the maintenance of telomere structure and function. It is highly expressed in different cancer types and it contributes to cancer progression. To date, anti-cancer strategies to target TRF2 remain a challenge. Here, we developed a miRNA-based approach to reduce TRF2 expression. By performing a high-throughput luciferase screening of 54 candidate miRNAs, we identified miR-182-3p as a specific and efficient post-transcriptional regulator of TRF2. Ectopic expression of miR-182-3p drastically reduced TRF2 protein levels in a panel of telomerase- or Alternative Lengthening of Telomeres (ALT)-positive cancer cell lines. Moreover, miR-182-3p induced DNA damage at telomeric and pericentromeric site

SUBMITTER: Roberto Dinami 

PROVIDER: S-SCDT-10_15252-EMMM_202216033 | biostudies-other |

REPOSITORIES: biostudies-other