Exploiting spatial dimensions to enable parallelized continuous directed evolution

ABSTRACT: Current strategies to improve the throughput of continuous directed evolution technologies often involve complex mechanical fluid-controlling system or robotic platforms, which limits their popularization and application in general laboratories. Inspired by our previous study on bacterial range expansion, in this study, we report a system termed SPACE for rapid and extensively parallelizable evolution of biomolecules by introducing spatial dimensions into the landmark phage-assisted continuous evolution system. Specifically, M13 phages and chemotactic Escherichia coli cells were closely inoculated onto a semi-solid agar. The phages came into contact with the expanding front of the bacterial range, and then co-migrated with the bacteria. This system leverages competition over space, wherein

SUBMITTER: Dr Ting Wei 

PROVIDER: S-SCDT-10_15252-MSB_202210934 | biostudies-other |

REPOSITORIES: biostudies-other