Differentiation-related epigenomic changes define clinically distinct keratinocyte cancer subclasses

ABSTRACT: Keratinocyte cancers (KC) are the most prevalent malignancies in fair-skinned populations, posing a significant medical and economic burden to health systems. KC originate in the epidermis and mainly comprise basal cell carcinoma (BCC) and cutaneous squamous cell carcinoma (cSCC). Here we combined single-cell multi-omics, transcriptomics and methylomics to investigate the epigenomic dynamics during epidermal differentiation. We identified ~3,800 differentially accessible regions between undifferentiated and differentiated keratinocytes, corresponding to regulatory regions associated with key transcription factors. DNA methylation at these regions defined AK/cSCC subtypes with epidermal stem cell- or keratinocyte-like features. Using cell type deconvolution tools and integration of bulk and

SUBMITTER: Dr Manuel Rodríguez-Paredes 

PROVIDER: S-SCDT-10_15252-MSB_202211073 | biostudies-other |

REPOSITORIES: biostudies-other