Endophilin-A regulates presynaptic Ca2+ influx and synaptic vesicle recycling in auditory hair cells

ABSTRACT: Ribbon synapses of cochlear inner hair cells (IHCs) operate with high rates of neurotransmission, yet, the molecular regulation of synaptic vesicle (SV) recycling at these synapses remains poorly understood. Here, we studied the role of endophilins-A1-3, endocytic adaptors with curvature-sensing and -generating properties, in mouse IHCs. Single-cell RT-PCR indicated the expression of endophilins-A1-3 in IHCs and immunoblotting confirmed the presence of endophilins-A1 and -A2 in the cochlea. Patch-clamp recordings from endophilin-A-deficient IHCs revealed a reduction of Ca2+-influx and exocytosis, which we attribute to decreased abundance of presynaptic Ca2+-channels and impaired SV replenishment. Slow endocytic membrane retrieval, thought to reflect clathrin-mediated endocytosis, was impai

SUBMITTER: Ms. Jana Kroll 

PROVIDER: S-SCDT-EMBOJ-2018-100116 | biostudies-other |

REPOSITORIES: biostudies-other