The Ulp2 SUMO Protease Promotes Transcription Elongation through Regulation of Histone Sumoylation

ABSTRACT: Many eukaryotic proteins are regulated by modification with the ubiquitin-like protein SUMO. This linkage is reversed by SUMO proteases, of which there are two in Saccharomyces cerevisiae, Ulp1 and Ulp2. SUMO-protein conjugation regulates transcription, but the roles of SUMO proteases in transcription remain unclear. We report that Ulp2 is recruited to transcriptionally active genes to control local polysumoylation. Mutant ulp2 cells show impaired association of RNA polymerase II (RNAPII) with, and diminished expression of, constitutively active genes and the inducible CUP1 gene. Ulp2 loss sensitizes cells to 6-azauracil, a hallmark of transcriptional elongation defects. We also describe a novel chromatin regulatory mechanism whereby histone-H2B ubiquitylation stimulates histone sumoylatio

SUBMITTER: Dr. Hong-Yeoul Ryu 

PROVIDER: S-SCDT-EMBOJ-2019-102003 | biostudies-other |

REPOSITORIES: biostudies-other