Bronchioalveolar stem cells are a main source for regeneration of distal lung epithelia

ABSTRACT: Bronchioalveolar stem cells (BASCs) are a potential source for lung regeneration, but direct in vivo evidence for a multipotential lineage contribution during homeostasis and disease is critically missing, since specific genetic labeling of BASCs has not been possible. We developed a novel cell tracing approach based on intein-mediated assembly of newly engineered split-effectors, allowing selective targeting of dual-marker expressing BASCs in the mouse lung. RNA sequencing of isolated BASCs demonstrate that BASCs show a distinct transcriptional profile, characterized by co-expression of bronchiolar and alveolar epithelial genes. We found that BASCs generate the majority of distal lung airway cells after bronchioalveolar damage but only moderately contribute to cellular turnover under home

SUBMITTER: Isabelle Salwig 

PROVIDER: S-SCDT-EMBOJ-2019-102099 | biostudies-other |

REPOSITORIES: biostudies-other