NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells

ABSTRACT: In most animal cells, mitotic spindle formation is mediated by coordination of centrosomal and acentrosomal pathways. At the onset of mitosis, centrosomes promote spindle bipolarization. However, the mechanism through which the acentrosomal pathways facilitate the establishment of spindle bipolarity in early mitosis is not completely understood. In this study, we show the critical roles of nuclear mitotic apparatus protein (NuMA) in the generation of spindle bipolarity in acentrosomal human cells. In acentrosomal human cells, we found that small microtubule asters containing NuMA formed at the time of nuclear envelope breakdown. In addition, these asters were assembled by dynein and the clustering activity of NuMA. Subsequently, NuMA organized the radial array of microtubules which incorpo

SUBMITTER: Dr. Takumi Chinen 

PROVIDER: S-SCDT-EMBOJ-2019-102378 | biostudies-other |

REPOSITORIES: biostudies-other