Absolute proteomic quantification reveals design principles of sperm flagellar chemosensation

ABSTRACT: Cilia serve as cellular antennae that translate sensory information into physiological responses. In the sperm flagellum, a single chemoattractant molecule can trigger a Ca2+rise that controls motility. The mechanisms underlying such ultra-sensitivity are ill-defined. Here, we determine by mass spectrometry the copy number of nineteen chemosensory signaling proteins in sperm flagella from the sea urchin Arbacia punctulata. Proteins are up to 1,000-fold more abundant than the free cellular messengers cAMP, cGMP, H+, and Ca2+. Opto-chemical techniques show that high protein concentrations kinetically compartmentalize the flagellum: within milliseconds, cGMP is relayed from the receptor guanylate cyclase to a cGMP-gated channel that serves as a perfect chemo-electrical transducer. cGMP is rap

SUBMITTER: Dr. U., Benjamin Kaupp 

PROVIDER: S-SCDT-EMBOJ-2019-102723P | biostudies-other |

REPOSITORIES: biostudies-other