Polarized sorting of Patched enables cytoneme-mediated Hedgehog reception in the Drosophila wing disc

ABSTRACT: Hedgehog (Hh) signal molecules play a fundamental role in development, adult stem cell maintenance and cancer. Hh can signal at a distance and we have proposed that its graded distribution across Drosophila epithelia is mediated by filopodia-like structures called cytonemes. Hh reception by Patched (Ptc) happens at discrete sites along presenting and receiving cytonemes, reminiscent of synaptic processes. Here we show that a vesicle fusion mechanism mediated by SNARE proteins is required for Ptc placement at contact sites. Transport of Ptc to these sites requires multi vesicular bodies (MVBs) formation via ESCRT machinery, in a manner different to that regulating Ptc/Hh lysosomal degradation after reception. These MVBs include extracellular vesicle (EV) markers and, accordingly, Ptc is det

SUBMITTER: Dr. Laura González-Méndez 

PROVIDER: S-SCDT-EMBOJ-2019-103629P | biostudies-other |

REPOSITORIES: biostudies-other