Phosphoproteomics reveals novel modes of function and inter-relationships among PIKKs in response to genotoxic stress

ABSTRACT: The DNA damage response (DDR) is a complex signaling network that relies on cascades of protein phosphorylation, which are initiated by three protein kinases of the family of PI3-kinase-related protein kinases (PIKKs): ATM, ATR and DNA-PK. ATM is missing or inactivated in the genome instability syndrome, ataxia-telangiectasia (A-T). The relative shares of these PIKKs in the response to genotoxic stress and the functional relationships among them are central questions in the genome stability field. We conducted a comprehensive phosphoproteomic analysis in human wild-type and A-T cells treated with the double-strand break-inducing chemical, neocarzinostatin, and validated the results with the targeted proteomic technique, selected reaction monitoring. We also matched our results with 34 pub

SUBMITTER: Prof. Yosef, Y Shiloh 

PROVIDER: S-SCDT-EMBOJ-2020-104400 | biostudies-other |

REPOSITORIES: biostudies-other