Liquid-Liquid Phase Separation Underpins the Formation of Replication Factories in Rotaviruses

ABSTRACT: RNA viruses induce the formation of subcellular organelles that provide microenvironments conducive to their replication. Here we show that replication factories of rotaviruses represent protein-RNA condensates that are formed via liquid-liquid phase separation of the viroplasm-forming proteins NSP5 and rotavirus RNA chaperone NSP2. Upon mixing, these proteins readily form condensates at physiologically relevant low micromolar concentrations achieved in the cytoplasm of virus-infected cells. Early infection stage condensates could be reversibly dissolved by 1,6-hexanediol, as well as propylene glycol that released rotavirus transcripts from these condensates. During the early stages of infection, propylene glycol treatments reduced viral replication and phosphorylation of the condensate-fo

SUBMITTER: Mr. Florian Geiger 

PROVIDER: S-SCDT-EMBOJ-2021-107711 | biostudies-other |

REPOSITORIES: biostudies-other