Single-cell transcriptomics of LepR-positive skeletal cells reveals heterogeneous stress-dependent stem and progenitor pools

ABSTRACT: Leptin receptor (LepR)-positive cells are key components of the bone marrow hematopoietic microenvironment, and highly enrich skeletal stem and progenitor cells that maintain homeostasis of the adult skeleton. However, the heterogeneity and lineage hierarchy within this population has been elusive. Using genetic lineage tracing and single-cell RNA-sequencing, we found that Lepr-Cre labels most bone marrow stromal cells and osteogenic lineage cells in adult long bones. Integrated analysis of Lepr-Cre-traced cells under homeostatic and stress conditions revealed dynamic changes of the adipogenic, osteogenic and periosteal lineages. Importantly, we discovered a Notch3+ bone marrow subpopulation that is slow-cycling and closely associated with the vasculatures, as well as key transcriptional n

SUBMITTER: Chunyang Mo 

PROVIDER: S-SCDT-EMBOJ-2021-108415 | biostudies-other |

REPOSITORIES: biostudies-other