Subcellular localization of Type VI secretion system assembly in response to cell-cell contact

ABSTRACT: Bacteria require a number of systems, including the type VI secretion system (T6SS), for interbacterial competition and pathogenesis. The T6SS is a large nanomachine that can deliver toxins directly across membranes of proximal target cells. Since major reassembly of T6SS is necessary after each secretion event, accurate timing and localization of T6SS assembly can lower the cost of protein translocation. Though critically important, mechanisms underlying spatiotemporal regulation of T6SS assembly remain poorly understood. Here we used super-resolution live-cell imaging to show that while Acinetobacter and Burkholderia thailandensis can assemble T6SS at any site, a significant subset of T6SS assemblies localizes precisely to the site of contact between neighboring bacteria. We identified a

SUBMITTER: Lin Lin 

PROVIDER: S-SCDT-EMBOJ-2021-108595 | biostudies-other |

REPOSITORIES: biostudies-other