Single-cell transcriptomics of suprachiasmatic nuclei reveal a Prokineticin-driven circadian network

ABSTRACT: Circadian rhythms in mammals are governed by the hypothalamic suprachiasmatic nucleus (SCN), in which 20,000 clock cells are connected together into a powerful time-keeping network. In the absence of network-level cellular interactions, the SCN fails as a clock. The topology and specific roles of its distinct cell populations (nodes) that direct network functions are, however, not understood. To characterise its component cells and network structure, we conducted single-cell sequencing of SCN organotypic slices and identified eleven distinct neuronal sub-populations across circadian day and night. We defined neuropeptidergic signalling axes between these nodes, and built neuropeptide-specific network topologies. This revealed their temporal plasticity, being upregulated in circadian day. T

SUBMITTER: Dr. Emma, Louise Morris 

PROVIDER: S-SCDT-EMBOJ-2021-108614 | biostudies-other |

REPOSITORIES: biostudies-other