PABP prevents the untimely decay of select mRNA populations in human cells

ABSTRACT: Gene expression is tightly regulated at the levels of both mRNA translation and stability. The poly(A)-binding protein (PABP) is thought to play a role in regulating these processes by binding the mRNA 3´ poly(A) tail and interacting with both the translation and mRNA deadenylation machineries. In this study we directly investigate the impact of PABP on translation and stability of endogenous mRNAs in human cells. Remarkably, our transcriptome-wide analysis only detects marginal mRNA translation changes in PABP-depleted cells. In contrast, rapidly depleting PABP alters mRNA abundance and stability, albeit non-uniformly. Otherwise stable transcripts, including those encoding proteins with constitutive functions, are destabilized in PABP-depleted cells. In contrast, many unstable mRNAs, incl

SUBMITTER: Mr. Sam Kajjo 

PROVIDER: S-SCDT-EMBOJ-2021-108650 | biostudies-other |

REPOSITORIES: biostudies-other