Ribosome slowdown triggers codon-mediated mRNA decay independently of ribosome quality control

ABSTRACT: The control of mRNA stability plays a central role in regulating gene expression patterns. Recent studies have revealed that codon composition in the open reading frame determines mRNA stability in multiple organisms. Based on genome-wide correlation approaches, this previously unrecognized role for the genetic code is attributable to the kinetics of the codon-decoding process by the ribosome. However, complementary experimental analyses are required to clarify the codon effects on mRNA stability and the related cotranslational mRNA decay pathways, for example those triggered by aberrant ribosome stalling. In the current study, we performed a set of reporter-based analyses to define codon-mediated mRNA decay and ribosome stall-dependent mRNA decay in zebrafish embryos. Our analysis showed

SUBMITTER: Prof. Yuichiro Mishima 

PROVIDER: S-SCDT-EMBOJ-2021-109256 | biostudies-other |

REPOSITORIES: biostudies-other