A conserved signaling pathway activates bacterial CBASS immune signaling in response to DNA damage

ABSTRACT: To protect themselves from the constant threat of bacteriophage (phage) infection, bacteria have evolved diverse immune systems including restriction-modification, CRISPR-Cas, and many others. Here we describe the discovery of a two-protein transcriptional regulator module associated with hundreds of CBASS immune systems, and demonstrate that this module drives expression of its associated CBASS system in response to DNA damage. We show that the helix-turn-helix transcriptional repressor CapH binds the promoter region of its associated CBASS system to repress transcription until it is cleaved by the metallopeptidase CapP. CapP is activated in vitro by single-stranded DNA, and in cells by DNA-damaging drugs. Together, CapH and CapP drive increased expression of their associated CBASS system

SUBMITTER: Ms Rebecca Lau 

PROVIDER: S-SCDT-EMBOJ-2022-111540 | biostudies-other |

REPOSITORIES: biostudies-other