Ttyh1 regulates embryonic neural stem cell properties by enhancing the Notch signaling pathway

ABSTRACT: Despite growing evidence linking Drosophila melanogaster tweety-homolog 1 (Ttyh1) to normal mammalian brain development and cell proliferation, its exact role has not yet been determined. Here, we show that Ttyh1 is required for the maintenance of neural stem cell (NSC) properties as assessed by neurosphere formation and in vivo analyses of cell localization after in utero electroporation. We find that enhanced Ttyh1-dependent stemness of NSCs is caused by enhanced ?-secretase activity resulting in increased levels of Notch intracellular domain (NICD) production, and activation of Notch targets. This is a unique function of Ttyh1 among all other Ttyh family members. Molecular analyses revealed that Ttyh1 binds to the regulator of ?-secretase activity Rer1 in the endoplasmic reticulum, and

SUBMITTER: Juwan Kim 

PROVIDER: S-SCDT-EMBOR-2017-45472V1 | biostudies-other |

REPOSITORIES: biostudies-other