Robotic platform for microinjection into single cells in brain tissue

ABSTRACT: Microinjection into single cells in brain tissue is a powerful technique to study and manipulate neural stem cells. However, such microinjection requires expertise and is a low-throughput process. We developed the 'Autoinjector', a robot that utilizes images from a microscope to guide a microinjection needle into tissue to deliver femtoliter volumes of liquids into single cells. The Autoinjector enables microinjection of hundreds of cells within a single organotypic slice, resulting in an overall yield that is an order of magnitude greater than manual microinjection. The Autoinjector successfully targets both apical progenitors (APs) and newborn neurons in the embryonic mouse and human fetal telencephalon. We used the Autoinjector to systematically study gap-junctional communication betwee

SUBMITTER: Gabriella, M Shull 

PROVIDER: S-SCDT-EMBOR-2019-47880V1 | biostudies-other |

REPOSITORIES: biostudies-other