TEFM regulates both transcription elongation and RNA processing in mitochondria

ABSTRACT: Regulation of replication and expression of mitochondrial DNA (mtDNA) is essential for cellular energy conversion via oxidative phosphorylation. The mitochondrial transcription elongation factor (TEFM) has been proposed to regulate the switch between transcription termination for replication primer formation and processive, near-genome length transcription for mtDNA gene expression. Here, we report that Tefm is essential for mouse embryogenesis and that levels of promoter-distal mitochondrial transcripts are drastically reduced in conditional Tefm-knockout hearts. In contrast, the promoter-proximal transcripts are much increased in Tefm knockouts, but they mostly terminate before the region where the switch from transcription to replication occurs, and consequently de novo mtDNA replicatio

SUBMITTER: Dr. Shan Jiang 

PROVIDER: S-SCDT-EMBOR-2019-48101-T | biostudies-other |

REPOSITORIES: biostudies-other