Wnt regulates amino acid transporter Slc7a5 constraining integrated stress response in mouse embryos

ABSTRACT: Amino-acids are essential for cellular metabolism and it is important to understand how nutrient supply is coordinated with changing energy requirements during embryogenesis. Here we show that the amino-acid transporter Slc7a5/Lat1 is highly expressed in tissues undergoing morphogenesis and that Slc7a5-null mouse embryos have profound neural and limb-bud outgrowth defects. Slc7a5-null neural tissue exhibited aberrant mTORC1 activity and cell proliferation; transcriptomics, protein phosphorylation and apoptosis analyses further indicated induction of the integrated stress response as a potential cause of observed defects. The pattern of stress-response gene expression induced in Slc7a5-null embryos was also detected at low-level in wildtype embryos and identified stress-vulnerability specif

SUBMITTER: Dr. Nadège Poncet 

PROVIDER: S-SCDT-EMBOR-2019-48469-T | biostudies-other |

REPOSITORIES: biostudies-other