High grade mesenchymal pancreatic ductal adenocarcinoma drives stromal deactivation through CSF-1

ABSTRACT: Pancreatic ductal adenocarcinoma (PDAC) is characterized by an abundance of stroma. Multiple molecular classification efforts have identified a mesenchymal tumor subtype that is consistently characterized by high-grade growth and poor clinical outcome. The relation between PDAC stroma and tumor subtypes is still unclear. Here, we aimed to identify how PDAC cells instruct the main cellular component of stroma, the pancreatic stellate cells (PSCs). We found in primary tissue that high-grade PDAC has reduced collagen deposition compared to low-grade PDAC. Xenografts and organotypic co-cultures established from mesenchymal-like PDAC cells features reduced collagen and activated PSC content. Medium transfer experiments using a large set of PDAC cell lines revealed that mesenchymal-like PDAC cel

SUBMITTER: Mrs. Anne Steins 

PROVIDER: S-SCDT-EMBOR-2019-48780V1 | biostudies-other |

REPOSITORIES: biostudies-other