A cell atlas of adult muscle precursors uncovers early events in fiber-type divergence in Drosophila

ABSTRACT: In Drosophila, the wing disc-associated muscle precursors cells give rise to the fibrillar indirect flight muscles (IFM) and the tubular direct flight muscles (DFM). To understand early transcriptional events underlying this muscle diversification, we performed single cell RNA-sequencing experiments and built a cell atlas of myoblasts associated with third instar larval wing disc. Our analysis identified distinct transcriptional signatures for IFM and DFM myoblasts that underlie the molecular basis of their divergence. The atlas further revealed various states of differentiation of myoblasts, thus illustrating previously unappreciated spatial and temporal heterogeneity among them. We identified and validated novel markers for both IFM and DFM myoblasts at various states of differentiation,

SUBMITTER: Maria, Paula Zappia 

PROVIDER: S-SCDT-EMBOR-2019-49555-T | biostudies-other |

REPOSITORIES: biostudies-other