N(6)-methyladenosine-binding protein YTHDF1 suppresses EBV replication and promotes EBV RNA decay

ABSTRACT: N6-methyladenosine (m6A) modification of mRNA mediates diverse cellular and viral functions. Infection with Epstein-Barr virus (EBV) is causally associated with nasopharyngeal carcinoma (NPC), 10% of gastric carcinoma, and various B cell lymphomas, in which the viral latent and lytic phases both play vital roles. Here, we show that EBV transcripts exhibit differential m6A modification in human NPC biopsies, patient-derived xenograft tissues, and cells at different EBV infection stages. m6A-modified EBV transcripts are recognized and destabilized by the YTHDF1 protein, which leads to the m6A-dependent suppression of EBV infection and replication. Mechanistically, YTHDF1 hastens viral RNA decapping and mediates RNA decay by recruiting RNA degradation complexes, including ZAP, DDX17, and DCP2

SUBMITTER: Dr. Tian-Liang Xia 

PROVIDER: S-SCDT-EMBOR-2020-50128V1 | biostudies-other |

REPOSITORIES: biostudies-other