Overlapping Roles for PLK1 and Aurora A During Meiotic Centrosome Biogenesis in Mouse Spermatocytes

ABSTRACT: Establishment of bipolar spindles during meiotic divisions ensures faithful chromosome segregation to prevent gamete aneuploidy. We analyzed centriole duplication, as well as centrosome maturation and separation during meiosis I and II using mouse spermatocytes. The first round of centriole duplication occurs during early prophase I, then centrosomes mature and begin to separate by the end of prophase I to prime formation of bipolar metaphase I spindles. A second round of centriole duplication occurs at late anaphase I, and, subsequently, centrosome separation coordinates bipolar segregation of sister chromatids during meiosis II. Using a germ-cell specific conditional knockout strategy, we show that Polo-like kinase 1 and Aurora A kinase are required for centrosome maturation and separati

SUBMITTER: Stephen, R Wellard 

PROVIDER: S-SCDT-EMBOR-2020-51023V1 | biostudies-other |

REPOSITORIES: biostudies-other