Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture

ABSTRACT: Nitrate commands genome-wide gene expression changes that impact metabolism, physiology, plant growth and development. In an effort to identify new components involved in nitrate responses in plants, we analyze the Arabidopsis thaliana root phosphoproteome in response to nitrate treatments via liquid chromatography coupled to tandem mass spectrometry. 176 phosphoproteins show significant changes at 5 min or 20 min after nitrate treatments. Proteins identified by 5 min include signaling-components such as kinases or transcription factors. In contrast, by 20 min, proteins identified were associated with transporter activity or hormone metabolism functions, among others. The phosphorylation profile of NITRATE TRANSPORTER 1.1 (NRT1.1) mutant plants was significantly altered as compared to wild

SUBMITTER: Dr. Andrea Vega 

PROVIDER: S-SCDT-EMBOR-2020-51813V1 | biostudies-other |

REPOSITORIES: biostudies-other