CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment

ABSTRACT: Progression through the cell cycle is driven by cyclin dependent kinases that control gene expression, orchestration of mitotic spindle and cell division. To identify new regulators of the cell cycle, we performed transcriptomic analysis of human non-transformed cells expressing a Fluorescent Ubiquitination-based Cell Cycle Indicator and identified 701 transcripts differentially expressed in G1 and G2 cells. Family with sequence similarity 110 member A (FAM110A) protein is highly expressed in G2 cells and localized at mitotic spindle and spindle poles during mitosis. Depletion of FAM110A impairs chromosomal alignment, delays metaphase-to-anaphase transition and affects spindle positioning. Using mass spectrometry and immunoprecipitation, we identified casein kinase I (CK1) in complex with

SUBMITTER: Cecilia, Aquino Perez 

PROVIDER: S-SCDT-EMBOR-2020-51847V1 | biostudies-other |

REPOSITORIES: biostudies-other