Constricted migration is associated with stable 3D genome structure differences in cancer cells

ABSTRACT: To spread from a localized tumor, metastatic cancer cells squeeze through constrictions that cause major nuclear deformations. Since chromosome structure affects nucleus stiffness, gene regulation and DNA repair, here we investigate the relationship between 3D genome structure and constricted migration in cancer cells. Using melanoma (A375) cells, we identify phenotypic differences in cells that have undergone multiple rounds of constricted migration. These cells display a stably higher migration efficiency, elongated morphology, and differences in the distribution of Lamin A/C and heterochromatin. Hi-C experiments reveal differences in chromosome spatial compartmentalization specific to cells that have passed through constrictions and related alterations in expression of genes associated

SUBMITTER: Ms. Rosela Golloshi 

PROVIDER: S-SCDT-EMBOR-2020-52149-T | biostudies-other |

REPOSITORIES: biostudies-other