HP1gamma binding pre-mRNA intronic repeats modulates RNA splicing decisions

ABSTRACT: HP1 proteins are best known as markers of heterochromatin and gene silencing. Yet, they are also RNA-binding proteins and the HP1gamma/Cbx3 family member is present on transcribed genes together with RNA polymerase II, where it regulates co-transcriptional processes such as alternative splicing. To gain insight in the role of the RNA binding activity of HP1gamma in transcriptionally active chromatin, we have captured and analyzed RNAs associated with this protein. We find that HP1gamma is specifically targeted to hexameric RNA motifs and coincidentally transposable elements of the SINE family. As these elements are abundant in introns, while essentially absent from exons, the HP1gamma RNA association tethers unspliced pre-mRNA to chromatin via the intronic regions and limits the usage of intronic cryptic splice sites. Thus, our data unveil novel determinants in the relationship between chromatin and co-transcriptional splicing

SUBMITTER: Dr. Christophe Rachez 

PROVIDER: S-SCDT-EMBOR-2020-52320-T | biostudies-other |

REPOSITORIES: biostudies-other