H2A.B is a cancer/testis factor involved in activation of ribosome biogenesis in Hodgkin Lymphoma

ABSTRACT: Testis-specific regulators of chromatin function are commonly expressed in human cancers, but their roles are poorly understood. Examination of 81 primary Hodgkin Lymphoma (HL) samples showed that the ectopic expression of the eutherian testis-specific histone variant H2A.B is an inherent feature of HL. In experiments using two HL-derived cell lines derived from different subtypes of HL, H2A.B knockdown inhibited cell proliferation. H2A.B was enriched in both the nucleoli of these HL cell lines and primary HL samples. We found that H2A.B enhanced ribosomal DNA (rDNA) transcription, was enriched at the rDNA promoter and transcribed regions, and interacted with RNA Pol I. Depletion of H2A.B caused the loss of RNA Pol I from rDNA chromatin. H2A.B was also required for high levels of ribosomal

SUBMITTER: Dr. Xuanzhao Jiang 

PROVIDER: S-SCDT-EMBOR-2021-52462V1 | biostudies-other |

REPOSITORIES: biostudies-other