CDC20 promotes bone formation via APC/C dependent ubiquitination and degradation of p65

ABSTRACT: The E3 ubiquitin ligase complex CDC20-activated Anaphase-promoting complex/Cyclosome (APC/CCDC20) plays a critical role in governing mitotic progression by targeting key cell-cycle regulators for degradation. Cell division cycle protein 20 homolog (CDC20), the co-activator of APC/C, is required for full ubiquitin ligase activity. In addition to its well-known cell-cycle related functions, we demonstrate that CDC20 plays an essential role in osteogenic commitment of bone marrow mesenchymal stromal/stem cells (BMSCs). Cdc20 conditional knockout mice exhibit decreased bone formation and impaired bone regeneration after injury. Mechanistically, we discovered a functional interaction between the WD40 domain of CDC20 and the DNA-binding domain of p65. Moreover, CDC20 promotes the ubiquitination

SUBMITTER: Yongsheng Zhou 

PROVIDER: S-SCDT-EMBOR-2021-52576V1 | biostudies-other |

REPOSITORIES: biostudies-other