PROTECTIVE ANTIGENIC SITES IDENTIFIED IN RESPIRATORY SYNCYTIAL VIRUS FUSION PROTEIN REVEALS IMPORTANCE OF p27 DOMAIN

ABSTRACT: Respiratory syncytial virus (RSV) vaccines primarily focused on surface fusion (F) protein are under development. Therefore, to identify RSV-F protective epitopes, we evaluated 14 antigenic sites recognized following primary human RSV infection. BALB/c mice were vaccinated with F peptides, F proteins, or RSV-A2, followed by rA2-Line19F challenge. F peptides generated binding antibodies with minimal in vitro neutralization titers. However, several F peptides (including Site II) reduced lung viral loads and lung pathology scores in animals, suggesting partial protection from RSV disease. Interestingly, animals vaccinated with peptides (aa 101-121 and 110-136) spanning the F-p27 sequence, which is only present in unprocessed F0 protein, showed control of viral loads with significantly reduce

SUBMITTER: Jeehyun Lee 

PROVIDER: S-SCDT-EMM-2020-13847-T | biostudies-other |

REPOSITORIES: biostudies-other