Stochastic transcription in the p53-mediated response to DNA damage is modulated by burst frequency

ABSTRACT: Discontinuous transcription has been described for different mammalian cell lines and numerous promoters. However, our knowledge of how the activity of individual promoters is adjusted by dynamic signaling inputs from transcription factor is limited. To address this question, we characterized the activity of selected target genes that are regulated by pulsatile accumulation of the tumor suppressor p53 in response to ionizing radiation. We performed time resolved measurements of gene expression at the single cell level by smFISH and used the resulting data to inform a mathematical model of promoter activity. We found that p53 target promoters are regulated by frequency modulation of stochastic bursting and can be grouped along three archetypes of gene expression. The occurrence of these arc

SUBMITTER: Ms Dhana Friedrich 

PROVIDER: S-SCDT-MSB-19-9068 | biostudies-other |

REPOSITORIES: biostudies-other