A dynamic, spatially periodic, micro-pattern of HES5 underlies neurogenesis in the mouse spinal cord

ABSTRACT: Ultradian oscillations of HES Transcription Factors (TFs) at the single cell level, enable cell state transitions. However, the tissue level organisation of HES5 dynamics in neurogenesis is unknown. Here, we analyse the expression of HES5 ex-vivo in the developing mouse ventral spinal cord and identify microclusters of 4-6 cells with positively correlated HES5 level and ultradian dynamics. These microclusters are spatially periodic along the dorsoventral axis and temporally dynamic, alternating between high and low expression with a supra-ultradian persistence time. We show that Notch signaling is required for temporal dynamics but not the spatial periodicity of HES5. Few Neurogenin-2 cells are observed per cluster, irrespective of high or low state, suggesting that the microcluster organi

SUBMITTER: Dr. Veronica Biga 

PROVIDER: S-SCDT-MSB-20-9902 | biostudies-other |

REPOSITORIES: biostudies-other