Transcriptomic characterisation of haematopoietic stem and progenitor cells from adult human homeostatic (non-mobilised) and mobilised peripheral blood

ABSTRACT: Haematopoietic stem and progenitor cells (HSPCs), the precursors of all blood cells, reside predominantly in the bone marrow. Yet, a small proportion (<1%) of phenotypic HSPCs circulates through peripheral blood (PB) at any given time. In contrast, mobilising agents, such as G-CSF can increase HPSC content in PB and are widely used to source HSPCs for clinical transplantation. To date, the detailed cellular and molecular composition of non-mobilised and mobilised PB (mPB) HSPCs in adult humans remains very poor. Here, we characterized the single-cell transcriptomes of >51,000 adult human HSPCs within non-mobilised PB from six healthy donors as well as of >25,000 HSPCs isolated from four mPB samples. We combined this analysis with single-cell functional analysis using most immature haematop

ORGANISM(S): Homo sapiens (human)

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PROVIDER: S-SUBS10 | biostudies-other |

SECONDARY ACCESSION(S): SAMEA7178002

REPOSITORIES: biostudies-other