Single-cell transcriptional landscape of human embryonic limb development

ABSTRACT: How the limb bud gives rise to the limb is a classic paradigm of model organism developmental biology, which is uncharted in human, and incompletely understood at the molecular and cellular levels. Here, we analyze the transcriptomes of 27,426 cells from 5 human embryonic limbs between 5 and 8 weeks post-conception to build a high-resolution single-cell transcriptome atlas of embryonic limb development. We developed a new toolkit, “PLOGS”, to visualize genes guiding cell fate decisions. Using this approach, we decipher the developmental trajectories of the major mesodermal lineages, and identify regulatory factors in limb development. For the skeletal muscle lineage, we show how Pax3+ cells directly differentiate towards embryonic myocytes, as well as give rise to the Pax7+ stem cell reser

ORGANISM(S): Homo sapiens

SUBMITTER: Sarah A Teichmann 

PROVIDER: S-SUBS5 | biostudies-other |

SECONDARY ACCESSION(S): SAMEA6735958

REPOSITORIES: biostudies-other