Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CS5_UL11_OXstress_HepG2_BAC-GFP_SRXN1_48hTP_v1

ABSTRACT:

ORGANISM(S): human

SUBMITTER: Bob van de Water

PROVIDER: S-TOXR1012 | biostudies-other |

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Similar Datasets

MEMA AU565 Staining Set 100 (Lapatinib treatment)

Project description:AU565 cells were grown in media with Lapatinib in 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(Basal lineage), KRT19 (luminal lineage) and EdU (S phase activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1469 | LINCS

MEMA HMEC122L Staining Set C

Project description: Not available

2016-11-10 | LDS-1463 | LINCS

LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 4 of 6: Nuclear fluorescent intensity measurements at 48 hr.

Project description: Not available

2017-05-12 | LDS-1405 | LINCS

MEMA HMEC122L Staining Set 4

Project description:HMEC122L cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(basal lineage), KRT19(luminal lineage) and EdU(Active S phase). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1431 | LINCS

MEMA MCF10A Staining Set 1

Project description:MCF10A cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), phalloidin (actin), CellMask (cytoplasm) and MitoTracker (metabolic activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1443 | LINCS

LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 13 of 15: Time-dependent dose-response metrics for biological replicate 1.

Project description: Not available

2017-05-12 | LDS-1420 | LINCS

Cell Painting morphological profiling assay

Project description:This dataset comprises L1000 and image-based cell-morphology profiles for small-molecule perturbations. Profiles were measured for ~18,000 compounds derived from diversity-oriented synthesis activities at the Broad Institute Center for the Science of Therapeutics, ~10,000 compounds selected from the Molecular Libraries Small-Molecule Repository (MLSMR), and ~2,000 known bioactive compounds. Each of the three subsets was selected to be structurally diverse. MLSMR compounds were further selected to have diverse biological performance. Imaging profiles were measured for a total of ~30,000 compounds. We used a "cell-painting" assay to stain seven major cellular components with fluorescent dyes followed by automatic image capture and analysis to extract shape, texture, and intensity parameters. The imaging assay protocol has been published by Gustafsdottir SM et al. L1000 profiles were measured for ~20,000 compounds. All experiments were performed with U-2 OS cells. Among other activities, we have analyzed these data using cheminformatics approaches to derive structure-based associations with patterns of performance in the profiles. The generation of this dataset was supported by the Molecular Libraries Program of the National Institutes of Health (U54-HG005032). Data made available as part of this project includes L1000 data and image-based cell-morphology profiles for small-molecule perturbations. Please follow the steps here to download the data: https://www.pnas.org/content/111/30/10911

2014-05-30 | LDS-1195 | LINCS

Growth rate-corrected (GR) dose-response metric signatures across a panel of 71 breast cancer cell lines treated with a library of small molecule and antibody perturbagens. Dataset 4 of 4: Median, upper quartile, and lower quartile GR metrics per perturbagen class.

Project description: Not available

2017-02-01 | LDS-1365 | LINCS

Growth rate-corrected (GR) dose-response metric signatures across a panel of 71 breast cancer cell lines treated with a library of small molecule and antibody perturbagens. Dataset 3 of 4: Median, upper quartile, and lower quartile GR metrics per perturbagen.

Project description: Not available

2017-07-20 | LDS-1364 | LINCS

MEMA MCF7 Staining Set 1

Project description:Cell cycle and nuclear state imaging readouts for MCF7 cells treated with ligand and ECMp combinations to explore the role of microenvironmental signals in cancer growth.

2016-03-09 | LDS-1299 | LINCS

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data