Project description:AU565 cells were grown in media with DMSO as a control in 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(Basal lineage), KRT19 (luminal lineage) and EdU (S phase activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1465 | LINCS

MEMA MCF10A Staining Set 2 no H3

Project description: Not available

2016-11-10 | LDS-1453 | LINCS

MEMA HMEC240L Staining Set 4

Project description:HMEC240L cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(basal lineage), KRT19(luminal lineage) and EdU(Active S phase). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1439 | LINCS

LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 2 of 15: End-point cell counts and normalized growth rate inhibition values for all technical replicates of biological replicate 2.

Project description: Not available

2017-05-12 | LDS-1409 | LINCS

Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 2 of 4)

Project description: Not available

2017-01-20 | LDS-1369 | LINCS

LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 2 of 9: Cell count and normalized growth rate inhibition values for biological replicate 2

Project description: Not available

2017-02-07 | LDS-1374 | LINCS

Dataset Information

WP7_UL32_DNAdamage_HepG2_BAC-GFP_BTG2_24hTP

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