Unknown

Dataset Information

0

Global 3' RACE in yeast and humans


ABSTRACT: Mpn1 proteins are evolutionarily conserved exonucleases that modify spliceosomal U6 small nuclear RNAs (snRNAs) post-transcriptionally. Mutations in the human MPN1 gene are associated to the genodermatosis Clericuzio-type poikiloderma with neutropenia (PN). Mpn1 deficiency leads to aberrant U6 3M-bM-^@M-^Y end processing and accelerated U6 decay through unknown molecular mechanisms. Here we show that in mpn1M-NM-^T fission yeast cells U6 is barely bound by the protective Lsm2-8 complex, undergoes extensive oligoadenylation and is degraded by the nuclear RNA exonuclease Rrp6 independently of the poly(A) polymerase Cid14/Trf4. Mpn1 processes U6 in a spliceosome-dependent manner, as mutant U6 molecules that fail to join the spliceosome are not substrates for Mpn1. Moreover, human U6atac, the U6-like snRNA of the minor spliceosome, is a novel substrate for hMpn1. We unveil mechanistic details of a new U6 degradation pathway and further corroborate the notion that inefficient canonical and minor pre-mRNA splicing promotes PN. The 3' termini of total RNA containing 2'3' cyclic phosphate from Mpn1-proficient and deficient human and yeast cells was sequenced

ORGANISM(S): Homo sapiens

PROVIDER: E-GEOD-60197 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| E-GEOD-60196 | BioStudies
| E-GEOD-60195 | BioStudies
2014-08-08 | E-GEOD-60196 | ArrayExpress
2014-08-08 | E-GEOD-60197 | ArrayExpress
2014-08-08 | E-GEOD-60195 | ArrayExpress
| E-MTAB-1197 | BioStudies
2012-07-27 | E-MTAB-1197 | ArrayExpress
| S-EPMC3069503 | BioStudies
| S-EPMC7512442 | BioStudies
| S-EPMC6265477 | BioStudies