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Transcription profiling of Arabidopsis wild type Columbia-0 and mutant gh3.5-1D in response to pathogen Pst DC3000(avrRpt2)


ABSTRACT: To reveal the underlying molecular mechanism of GH3.5 action in modulating the SA and auxin pathways, we performed transcriptional profiling of gh3.5-1D plants after infection with or without Pst DC3000(avrRpt2) on a global scale using the Affymetrix Arabidopsis ATH1 GeneChip Experiment Overall Design: Each 3 leaves of plants of 5-week-old wild type Columbia-0 and mutant gh3.5-1D (heterozygous) were inoculated with Pst DC3000(avrRpt2)at 105 cfu mL-1. Leaves were harvested at 0 h (uninoculated) and 48 hours after inoculation. Three biological repeats were performed on Columbia-0 (wild-type, Col-0) and gh3.5-1D (mutant) after infection with or without Pst DC3000(avrRpt2), respectively.

SUBMITTER: Li Qun 

PROVIDER: E-GEOD-6556 | BioStudies | 2008-06-14

SECONDARY ACCESSION(S): GSE6556

REPOSITORIES: biostudies

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