LncRNA and mRNA expression profiles during osteoclastogenesis
ABSTRACT: Total RNA were extracted from osteoclasts differentiated from RAW264.7 cells at different stages: Monocytes (0h), Pre-osteoclasts (24h), Mature osteoclasts (72h) and activated osteoclasts (96h). LncRNA and mRNA expression profiles were decided by microarray. RAW264.7 cells were cultured with RANKL (100ng/ml) and M-CSF (50ng/ml) for 0h, 24h, 72h and 96h. TRAP stain, FAK stain, pit formation assay, fusion assay and q-PCR were performed to validate the in vitro model.
ORGANISM(S): Mus musculus
PROVIDER: E-GEOD-72478 | BioStudies |