Unknown

Dataset Information

0

Genome-wide binding profiles of Sp5 in differentiation mouse embryonic stem cells


ABSTRACT: We have determined the genome-wide binding profile of the transcription factor Sp5. Flag-tagged Sp5 was targeted to the HPRT locus in A2Lox.cre ES cells to allow for Doxycycline inducible expression. ES cells were cultured as embryoid bodies for 2 days to prime them for germ layer differentiation. Cells were then treated with Doxycycline for 24 hrs. We found that Flag-Sp5 prefentially bound to promoters associatd with several growth factor signalling pathways, but most prominently with the Wnt/beta-catenin pathway to selectively promote mesoderm differentiation over neural. These data implicate Sp5 as new regulator of Wnt/beta-catenin target expression to promotes ES cell differentation into the mesoderm lineage. Examination of Sp5 transcription factor binding in differentiating embryonic stem cells.

ORGANISM(S): Mus musculus

PROVIDER: E-GEOD-72989 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

2016-04-01 | E-GEOD-72989 | ArrayExpress
2016-04-01 | GSE72989 | GEO
| E-GEOD-73082 | BioStudies
2016-04-01 | GSE73082 | GEO
2016-04-01 | E-GEOD-73082 | ArrayExpress
| E-GEOD-73083 | BioStudies
| S-EPMC5777634 | BioStudies
| S-EPMC4822596 | BioStudies
| S-EPMC4732286 | BioStudies
| S-EPMC5647328 | BioStudies