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14028s:rpoH_heat_shock_37C_RNA

ABSTRACT: Gene expression in Salmonella enterica sv Typhimurium 14028s rpoH mutant strain IB314 after heat shock to 37C

SUBMITTER: Steffen Porwollik

PROVIDER: E-GEOD-809 | BioStudies | 2010-06-09

SECONDARY ACCESSION(S): GSE809

REPOSITORIES: biostudies

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14028s:rpoH_heat_shock_37C_RNA

Project description:Gene expression in Salmonella enterica sv Typhimurium 14028s rpoH mutant strain IB314 after heat shock to 37C

2010-06-09 | E-GEOD-809 | ArrayExpress

The nsrR regulon in Salmonella Typhimurium 14028s

Project description:In this study, we have defined the NsrR regulon in Salmonella enterica sv. Typhimurium 14028s using a transcriptional microarray. Wild-type and nsrR mutant S. Typhimurium were grown aerobically to early log-phase (OD600~0.5) at 37C in LB medium. Total RNA was isolated from three independent cultures of both strains and interrogated on a PCR product array representing almost all ORFs. Overall design: Three biological replicates of wt and nsrR mutant total RNAs, Cy3 and Cy5 dye swapped, were hybridized onto six PCR product arrays in two channels.

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SL1344_heat_shock_37C_RNA

Project description:Gene expression in Salmonella enterica sv Typhimurium SL1344 after heat shock to 37C

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Salmonella enterica serovar Typhimurium ATCC 14028S is tolerant to plant defenses triggered by the flagellin receptor FLS2.

Project description:Salmonellosis outbreaks associated with sprouted legumes have been a food safety concern for over two decades. Despite evidence that Salmonella enterica triggers biotic plant defense pathways, it has remained unclear how plant defenses impact Salmonella growth on sprouted legumes. We used Medicago truncatula mutants in which the gene for the flagellin receptor FLS2 was disrupted to demonstrate that plant defenses triggered by FLS2 elicitation do not impact the growth of Salmonella enterica serovar Typhimurium ATCC 14028S. As a control, we tested the growth of Salmonella enterica serovar Typhimurium LT2, which has a defect in rpoS that increases its sensitivity to reactive oxygen species. LT2 displayed enhanced growth on M. truncatula FLS2 mutants in comparison to wild-type M. truncatula. We hypothesize that these growth differences are primarily due to differences in 14028S and LT2 reactive oxygen species sensitivity. Results from this study show that FLS2-mediated plant defenses are ineffective in inhibiting growth of Salmonella entrica 14028S.

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Project description:Gene content in Salmonella enterica sv Typhimurium 14028s after treatment with 2mM hydrogen peroxide Keywords: time-course

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opdA, a Salmonella enterica serovar Typhimurium gene encoding a protease, is part of an operon regulated by heat shock.

Project description:The opdA (prlC) gene of Salmonella enterica serovar Typhimurium and Escherichia coli encodes the metalloprotease oligopeptidase A (OpdA). We report that opdA is cotranscribed with a downstream open reading frame, yhiQ. Transcription of this operon is induced after a temperature shift (30 to 42 degrees C), and this induction depends on the heat shock sigma factor encoded by the rpoH (htpR) gene.

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Salmonella 14028s AraC ChIP-Seq

Project description:We mapped the genome-wide binding of C-terminally FLAG-tagged AraC in S. enterica subsp. enterica serovar Typhimurium strain 14028s using ChIP coupled with deep sequencing (ChIP-seq). We identified five putative target loci for AraC: upstream of araB/araC, araE, araJ, STM14_0178, and within sseD.

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Role of DksA in nitrosative stress

Project description:Transcriptional profiles of wt and dksA minus Salmonella enterica sv Typhimurium 14028S in E salts minimal medium in response to 5 mM DETANONOate for 30 min Total RNA was harvested from three biological replicates of wt and dksA mutant cultures exposed or unexposed to 5mM DETANONOate for 30min in E salts minimal medium.

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SL1344_heat_shock_42C_RNA

Project description:Gene expression in Salmonella enterica sv Typhimurium SL1344 after heat shock to 42C

2010-06-09 | E-GEOD-808 | ArrayExpress

Transcriptomic data of Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S treated with novobiocin.

Project description:In enteric bacteria, DNA supercoiling is highly responsive to environmental conditions. Host specific features of environment serve as cues for the expression of genes required for colonization of host niches via changing supercoiling [1]. It has been shown that substitution at position 87 of GyrA of Salmonella enterica str. SL1344 influences global supercoiling and results in an altered transcriptome with increased expression of stress response pathways [2]. Aminocoumarin antibiotics, such as novobiocin, can be used to relax supercoiling and alter the expression of supercoiling-sensitive genes. Meanwhile, Salmonella enterica demonstrates a significant resistance to this antibiotic and relatively small variability of supercoiling in response to the growth phase, osmotic pressure, and novobiocin treatment. Here we present for the first time transcriptome data of Salmonella enterica subsp. Enterica serovar Typhimurium str. 14028S grown in the presence of novobiocin. These data will help identify genes involved in novobiocin resistance and adaptation processes associated with torsion perturbations in S. enterica. Cleaned FASTQ files for the RNA-seq libraries are deposited in the NCBI Sequence Read Archive (SRA, Identifier: SRP239815) and have been assigned BioProject accession PRJNA599397.

2020-01-01 | S-EPMC7044638 | BioStudies

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