Dataset Information


Polysomal and total RNA in controls and after 10 min global brain ischemia and 8 hours of reperfusion

ABSTRACT: Complete global brain ischemia (CGBI) and reperfusion occur following resuscitation from cardiac arrest. Different brain neurons are selectively vulnerable to CGBI: pyramidal neurons of hippocampal CA3 survive 10 min CGBI but those of CA1 die at 3 days following 10 min CGBI. CA3 neurons are expected to have more robust stress responses and repair responses than CA1 neurons. We used microarrays to compared total and polysome-bound mRNAs in CA1 and CA3 at 8 hr reperfusion after 10 min CGBI in Long Evans male rats to ascertain differences in total vs polysome-bound gene expression. Male Long Evans rats were subjected to (1) sham operation (non-ischemic control, NIC) or normothermic CGBI of 10 min followed by 8 hr reperfusion (8R). Hippocampal CA1 and CA3 were dissected. n = 5 CA1 or CA3 were pooled to give a single replicate and there were 3 or 4 replicates per group. Post-mitochondrial supernatant (PMS) was prepared. Twenty percent of PMS was TRIzol extracted to give total RNA. The remainder was run on a 20% sucrose pad to isolate polysome pellets, which were also TRIzol extracted to give polysome RNA. Total and polysome RNA were then run on Affymetrix Rat Gene 2.0 microarrays.

ORGANISM(S): Rattus norvegicus

PROVIDER: E-GEOD-82146 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

2016-06-02 | E-GEOD-82146 | ArrayExpress
| S-EPMC3864748 | BioStudies
| S-EPMC5453468 | BioStudies
2016-07-07 | PXD004078 | Pride
| S-EPMC5242414 | BioStudies
| S-EPMC5567420 | BioStudies
| S-EPMC5373668 | BioStudies
2016-01-01 | S-EPMC5101406 | BioStudies
| S-EPMC4556686 | BioStudies
| S-EPMC3620228 | BioStudies