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Transcription profiling of Mycobacterium tuberculosis H37Rv wild type and deletion mutants for mce3R gene to investigate the expression of a regulon involved in lipid metabolism


ABSTRACT: Four cultures (four biological replicates) of each of two strains, wild type H37Rv and a deletion mutant for mce3R gene, were grown in in Middlebrook 7H9 medium supplemented with 0.05% Tween 80, or Middlebrook 7H11, supplemented with albumin 0.5%, dextrose 0.4%, and 0.5% glycerol (M7H9-AD-G).
Total RNA was extracted from each culture, dyed with Cy5 and hybridised onto two separated microarrays (technical duplicates).
In the second channel a mix of genomic DNA from strains H37Rv and AF2122 was dyed with CY3 and used as a common control in all the microarrays.
An statistical analysis of microarray data was performed to detect genes with differential expression between genotypes.

SUBMITTER: Marcelo Soria 

PROVIDER: E-MEXP-2202 | BioStudies | 2009-06-17

REPOSITORIES: biostudies

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