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329

RNA-seq of metabolically labelled RNA by 4-thiouracil from mouse tissues


ABSTRACT: To identify cell type-specific RNA without cell sorting, mice expressing UPRT in a specific cell type were exposed to 4-thiouracil to label RNA only in the UPRT-expressing cells. Total RNA was extracted from tissues and treated with iodoacetamide followed by RNA-seq library preparation. The reads generated from 4-thiouracil-containing transcripts should contain T to C mismatches as a result of 4-thiouracil incorporations and thus can be identified as the transcripts generated in the UPRT-expressing cells.

SUBMITTER: Wayo Matsushima 

PROVIDER: E-MTAB-6353 | BioStudies | 2018-07-09

SECONDARY ACCESSION(S): ERP106180

REPOSITORIES: biostudies

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